How does light obscuration work

This light blockage is termed obscuration. The detector measures this reduction in light intensity and using a calibration curve, processes the signal to determine the size of the particle. Light Obscuration is another widely accepted particle sizing technique in many fields. Numerous carrier fluids, both organic and inorganic can be used with the instrumentation. PTL has utilized Light Obscuration to solve numerous pharmaceutical and industrial problems for clients over the years.

Wiggenhorn, G. Winter, W. Jiskoot, W. Friess, A. Hawe, Particles in therapeutic protein formulations, Part 1: overview of analytical methods.

Hauptnavigation Analytical services Formulation development About us. Additional Services — Overview Physico-chemical characterization of lyophilizates Container closure integrity testing Basic formulation characterization Functional assay. Liquid formulations — Overview Liquid versus frozen-liquid Low-concentration versus high-concentration liquid formulation Formulation robustness studies. Stability and forced degradation studies — Overview ICH stability studies In-use stability Forced degradation studies Comparability studies.

Lyophilization process development — Overview Cycle design and optimization Robustness testing, scale-up and transfer Lyo equipment Physico-chemical characterization of lyophilizates. Light obscuration LO. Light obscuration LO is the compendial technique for the analysis of subvisible particles in parenteral and ophthalmic products.

Contact us. Light obscuration works by passing a dilute stream of particles in a liquid suspension between a light source and a detector. The detector measures the reduction in light intensity and employing a calibration curve, processes the signal to determine particle size. Light obscuration technique provides good results from one to several thousand microns for any kind of foreign material in a liquid. Widely used across a variety of pharmaceutical applications, light obscuration is recognized by the ISO and USP as the primary method for measuring the size and number of particles in a variety of samples including protein-based formulations and other sterile preparations.

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